Development of microsatellite markers for the assessment of genetic diversity in Leiopotherapon plumbeus Kner 1864
Issue Date
2017
Abstract
Microsatellite loci from a Philippine-endemic silver perch, Leiopotherapon plumbeus Kner 1864, were isolated, characterised and used in the development of simple sequence repeat (SSR) markers for genetic diversity analysis. Ten primer pairs were designed from a microsatellite-enriched library and polymorphic markers were used in the diversity assessment of populations from three Philippine lakes (Laguna de Bay, Taal Lake and Sampaloc Lake). Seven primers detected a total of 33 alleles and obtained polymorphism information content (PIC) values ranging from 0.12– 0.75. The overall Ho and uHe values across the populations were 0.247 and 0.494, respectively. Pairwise population FST values showed low but significant differentiation while unweighted pair group method with arithmetic mean (UPGMA) cluster analysis revealed a closer relationship between populations from Taal and Sampaloc lakes. The markers used in this study can be used to jumpstart genetic-based breeding programmes and conservation strategies to save the dwindling population of this species.
Source or Periodical Title
Asian Fisheries Science
ISSN
0116-6514
Volume
30
Page
70-86
Document Type
Article
College
College of Arts and Sciences (CAS)
Physical Description
illustrations, tables
Language
English
Subject
Genetic diversity, Leiopotherapon plumbeus, Microsatellite-enriched library, SSR markers
Recommended Citation
Manalang, A.P., Diaz, M.G.Q. (2017). Development of Microsatellite Markers for the Assessment of Genetic Diversity in Leiopotherapon plumbeus Kner 1864. Asian Fisheries Science, 30, 70-86. DOI: 10.33997/j.afs.2017.30.2.002.
Identifier
https://doi.org/10.33997/j.afs.2017.30.2002
Digital Copy
yes