The effects of soil sterilization, mycorrhizal inoculation, and rates of phosphorus on growth and survival of Kalopanax septemlobus microplants during the acclimatization period

Issue Date

1-2012

Abstract

In order to establish some cultural practices that can improve growth and survival of somatic embryo (SE)-derived microplants during the acclimatization period, Kalopanax septemlobus was uninoculated or inoculated with mycorrhizal fungi coded as AMM6 (a mixture of unidentified species of Glomus and Acaulospora collected in a closed mine tailing site in Bonghwa, Korea) during ex vitro and grown in oven-sterilized peat vermiculite medium. After 2 months, treated microplants were transferred into pots filled with the same medium amended with phosphorus fertilizer {0, 2, 4, 8, 16 and 32 mg P [as Ca(H2PO4)2·H2O] kg medium-1 coded as P0, P2, P4, P8, P16 and P32, respectively}. At this stage, inoculated plants were greener, with broader leaves and well-developed root systems and had higher survival than the uninoculated ones. After 6 months, inoculated plants were 54 % heavier than the uninoculated counterpart. In sterile medium, total dry weight of uninoculated plants was promoted at P8 and highest at P16. Total dry weight at P16 by uninoculated plants was attained at P4 by the mycorrhiza-inoculated counterpart. In non-sterile medium, total dry weight of inoculated plants was increased at P8. By contrast, uninoculated plants did not respond to the applied P rates. In conclusion, more SE-derived microplants survived and grew better in sterile medium. Maximum benefits from AMM6 was attained with applied 4 and 8 mg P kg medium-1 (P4-P8) in sterile and non-sterile medium, respectively. © 2012 Korean Society for Plant Biotechnology and Springer.

Source or Periodical Title

Plant Biotechnology Reports

ISSN

1863-5466

Volume

7

Issue

1

Page

71-82

Document Type

Article

Physical Description

illustrations, tables

Language

English

Subject

Acaulospora, Ex vitro, Glomus, In vitro, Kalopanax septemlobus, Somatic embryo

Identifier

doi:10.1007/s11816-012-0238-z.

Digital Copy

yes

Link to Full Text

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