Genomic and serological detection of bat coronavirus from bats in the Philippines
Issue Date
7-2012
Abstract
Bat coronavirus (BtCoV) is assumed to be a progenitor of severe acute respiratory syndrome (SARS)-related coronaviruses. To explore the distribution of BtCoVs in the Philippines, we collected 179 bats and detected viral RNA from intestinal or fecal samples by RT-PCR. The overall prevalence of BtCoVs among bats was 29.6 %. Phylogenetic analysis of the partial RNA-dependent RNA polymerase gene suggested that one of the detected BtCoVs was a novel alphacoronavirus, while the others belonged to the genus Betacoronavirus. Western blotting revealed that 66.5 % of bat sera had antibodies to BtCoV. These surveys suggested the endemic presence of BtCoVs in the Philippines. © 2012 Springer-Verlag.
Source or Periodical Title
Archives of Virology
ISSN
0304-8608
Volume
157
Issue
12
Page
2349-2355
Document Type
Article
Language
English
Subject
Antibody, Bat coronavirus, Bats, Coronavirus, SARS, Severe acute respiratory syndrome, Viruses, Zoonosis
Recommended Citation
Tsuda, S., Watanabe, S., Masangkay, J.S., Mizutani, T., Alviola, P., Ueda, N., Iha, K., Taniguchi, S., Fujii, H., Kato, K., Horimoto, T., Kyuwa, S., Yoshikawa, Y., Akashi, H. (2012). Genomic and serological detection of bat coronavirus from bats in the Philippines. Archives of Virology, 157 (12), 2349-2355. doi:10.1007/s00705-012-1410-z.
Identifier
doi:10.1007/s00705-012-1410-z.
Digital Copy
yes