Monitoring Cry1Ab susceptibility in Asian corn borer (Lepidoptera: Crambidae) on Bt corn in the Philippines

Creator

Edwin Alcantara, University of the Philippines Los Banos
Arnold Estrada, Monsanto Philippines Inc.
Victor Alpuerto, Monsanto Philippines Inc.
Graham Head, Monsanto Company

Issue Date

5-2011

Abstract

Between 2002 and 2004, collections of egg masses of Asian corn borer (ACB), Ostrinia furnacalis (Guenée) were made from corn-planting sites on the major Philippine islands of Luzon (Laguna, Pangasinan, Camarines Sur and Isabela provinces) and Mindanao (Bukidnon and South Cotabato provinces). The resulting neonates were bioassayed for susceptibility to Bacillus thuringiensis (Bt) Cry1Ab protein. The median lethal concentrations (LC50s) for the different collections ranged from 0.42 to 2.37 ng/cm2. The bioassay results suggest that Philippine corn borer populations were highly susceptible to Cry1Ab protein prior to the widespread deployment of Bt corn. The upper limit of the estimated LC99 (104 ng/cm2) from the pooled bioassay data was selected as a candidate diagnostic concentration and subsequently tested on eleven ACB populations. Results of the validation assays showed that the mortality response of all the tested ACB populations was higher than the expected mortality (99%). Therefore, the concentration of 104 ng/cm2 was used to monitor susceptibility in ACB populations in the Philippines. Monitoring of field populations during 2009 in areas where Bt corn had been grown for 3 years found some enhanced survival of neonates at the diagnostic concentration but progeny of the diagnostic-concentration survivors did not survive on Bt corn, indicating that ACB populations in the Philippines remain susceptible to Cry1Ab-containing Bt corn hybrids. © 2010 Elsevier Ltd.

Source or Periodical Title

Crop Protection

ISSN

0261-2194

Volume

30

Issue

5

Page

554-559

Document Type

Article

Physical Description

map, tables

Language

English

Subject

Diagnostic concentration, Diet surface-overlay assay, Ostrinia furnacalis

Recommended Citation

Alcantara, Edwin & Estrada, Arnold & Alpuerto, Victor & Head, Graham. (2011). Monitoring Cry1Ab susceptibility in Asian Corn Borer (Lepidoptera: Crambidae) on Bt corn in the Philippines. Crop Protection, 30 (5), 554-559. doi:10.1016/j.cropro.2010.12.019.

Identifier

doi:10.1016/j.cropro.2010.12.019.

Digital Copy

yes