Tier I testing of Cry1Ab protein on Micraspis (Fab.) (Coleoptera: Coccinellidae) and evaluation of its functional response to different prey types

Issue Date

10-2011

Abstract

Micraspis discolor (Fab.) is a ubiquitous and abundant predaceous insect in corn fields. hazard from exposure to Cry1Ab protein was evaluated by laboratory toxicity test using both maximum hazard dose and functional response assays. Mortality resulting from exposure to Cry1Ab protein was evaluated using artificial diet assays. Larvae and adults of M. discolor did not exhibit significant mortality after treatment for 7 days with Cry1Ab protein with concentration 20x higher than the concentration of Cry1Ab found in Bt corn pollen. The functional response to different prey types was evaluated in the laboratory by offering to M. discolor, that has been fed with Bt corn pollen, corn leaf aphid Rhopalosiphum maidis Fitch and Asian corn borer Ostrinia furnacalis Guenee in varying numbers or densities. The type of functional response reflected in each set of predation was analyzed by logistic regression followed by curve fitting to model equation describing a particular type of functional response. M. discolor exhibited a Type III functional response to all prey types tested which remained unaltered after ingestion of Bt corn pollen. Attack rates for all prey types were density-dependent which is typical of a type III functional response.

Source or Periodical Title

Philippine Entomologist

ISSN

0048-3753

Volume

25

Issue

2

Page

122-130

Document Type

Article

Frequency

semi-annually

Physical Description

graphs ; tables

Language

English

En – AGROVOC descriptors

COLEOPTERA; COCCINELLIDAE; BACILLUS THURINGIENS; PLANT PESTS; TESTING; EVALUATION; ACUTE TOXICITY; PEST RESISTANCE

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