Mutagenicity potential of the novel drug trichosetin estimated by using the rec assay and micronucleus test

Issue Date

12-2009

Abstract

Trichosetin, a novel tetramic acid antibiotic produced in dual culture of Trichoderma harzianum H14 and Catharanthus roseus callus, has been demonstrated to possess notable activity against methicillin-resistant Staphylococcus aureus (MRSA). However, before such antibiotic could be developed and used in clinical trials as a potential therapeutic agent against MRSA, extensive studies are needed to evaluate its safety. The present study attempts to examine both the DNAdamaging potential, as well as the chromosome-breaking effect, of trichosetin through the use of the rec assay and micronucleus test, respectively. In the rec assay, trichosetin was tested at three concentrations: 10 μg.mL -1 , 100 μg.mL -1 , and 1000 μg.mL -1 . In the micronucleus test, trichosetin was administered intraperitoneally to the mice at 10 mg.kg -1 , 50 mg.kg -1 , and 100 mg.kg -1 body weight. Both assays revealed that trichosetin is not mutagenic. Further toxicity and mutagenicity studies using higher dosage levels of trichosetin should be conducted in order to establish its safety as a therapeutic agent.

Source or Periodical Title

Philippine Journal of Science

ISSN

317683

Volume

138

Issue

2

Page

119-124

Document Type

Article

College

College of Arts and Sciences (CAS)

Language

English

Subject

Antibiotic, Dual culture, MRSA, Mutagenicity assay

Digital Copy

YES

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