Isolation and partial characterization of a lectin from the seeds of Artocarpus camansi blanco

Issue Date

5-2007

Abstract

The aim of this study is to isolate lectin from a seed which had no economical use. A lectin was isolated and purified from the seeds of Artocarpus camansi Blanco by extraction with phosphate buffered saline at pH 7.2. Further purification was done by precipitation with ammonium sulfate at 0-90% saturation. The dialyzed ammonium sulfate precipitate was subjected to gel filtration chromatography using Sephadex G-200 and fractions were tested for lectin activity. Native PAGE was employed to establish the homogeneity of the lectin positive fractions. Fractions with the same band were pooled and lyophilized. The purified lectin is non-blood type specific since it agglutinated all human blood types. It is blood group specific because it agglutinated blood from cow but not from swine. The purified lectin is a complete lectin since it agglutinated erythrocytes without trypsin treatment and addition of metal ions. However, trypsination of erythrocytes increases the agglutination activity. The isolated lectin did not show any specificity towards the ten sugars used in this study. The molecular weight of the purified lectin was estimated by using Sephadex G-200 and was found to be around 2,000 kDa since it eluted in the void volume. The lectin was found to be stable at 20-40°C. © 2007 Asian Network for Scientific Information.

Source or Periodical Title

Asian Journal of Plant Sciences

ISSN

16823974

Volume

6

Issue

5

Page

757-764

Document Type

Article

Language

English

Subject

Artocarpus camansi, Isolation, Lectin, Seed

Identifier

DOI: 10.3923/ajps.2007.757.764

Digital Copy

YES

Link to Full Text

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