Optimization of chitinase production by Serratia marcescens LMP42 (BIOTECH 1749) and its control of Rhizoctonia solani Kuhn under laboratory conditions

Abstract

Serratia marcescens LPM42 (BIOTECH 1749) was grown by batch fermentation under varied cultural conditions to produce chitinase. The amount of crude chitinase excreted in the culture medium was determined turbidimetrically through its ability to release N-acetylglucosamine (GlcNAc) after reaction with colloidal chitin obtained from crab shells. Incubation of the culture broth containing 5% (v/v) inoculum and 0.6% (v/v) colloidal chitin without Tween 80 at an initial pH of 7.0 for 48 h at room temperature with continuous shaking (158 rpm) were the conditions optimum for chitinase production of S. marcescens LPM42 (BIOTECH 1749) (0.004 units mL-1). Laboratory experiments to control the growth of Rhizoctonia solani Kuhn showed that soaking the sorghum seeds coated with fungal mycelia for 3 to 5 h in undiluted crude chitinase extract resulted in significant reduction in mycelial proliferation after 12 h of incubation. Complete inhibition of mycelial development was observed after 12 h soaking in undiluted crude chitinase extract. No growth was observed even when the incubation was extended for 48 h. These results demonstrated the potential of using even the crude form of the chitinase enzyme for the biocontrol of R. solani.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

259-265

Document Type

Article

Subject

Biocontrol, Chitin, Chitinase, Rhizoctonia solani, Serratia marcescens

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