Strain improvement of Aspergillus ficuum NRRL 3135 and optimization of fermentation conditions for phytase production
Abstract
Phytase activities of mycelial and spore colonies of Aspergillus ficuum NRRL 3135 irradiated with UV for 10 to 60 min were determined. Spore colonies exposed to UV for 30 min gave the highest phytase activity (74.81 U L -1), which is more than three-fold higher than that of the wild type. Among the fermentation media containing varying levels (1 - 4% w/v) of rice bran, cornstarch, cornmeal and soybean meal as substrates, the use of rice bran (3% and 4% levels) resulted in the highest phytase activity without significant differences between their values. Enzyme activities were measured when the following ingredients were added at different levels (w/v) to the medium containing 3% rice bran: glucose (0.5 to 3.0%), nitrogen as sodium nitrate (0.5 to 2.0%) and peptone (0.1 to 0.5%). Based on the results, the fermentation medium should contain 0.5% glucose, 0.5% sodium nitrate and 0.2% peptone. Its enzyme activity increased to 380.46 U mL-1. The initial pH of the medium optimum for enzyme production was 5.5.
Source or Periodical Title
Philippine Agricultural Scientist
ISSN
317454
Page
387-394
Document Type
Article
Subject
Aspergillus ficuum NRRL 3135, Enzyme activity, Phosphorus, Phytase
Recommended Citation
Capitan, Carmela T. and Zamora, Agnes F., "Strain improvement of Aspergillus ficuum NRRL 3135 and optimization of fermentation conditions for phytase production" (2021). Journal Article. 3152.
https://www.ukdr.uplb.edu.ph/journal-articles/3152