A polymerase chain reaction protocol for the detection of various geographical isolates of white spot virus

Abstract

Polymerase chain reaction (PCR) primers were designed based on the sequence of a cloned fragment of the white spot virus (WSV) genome and were used to detect at least four geographic isolates of WSV from both experimentally-and naturally-infected shrimp. In addition to high specificity, the one-step and two-step PCR protocols were determined to have sensitivities of 10-100 pg and 100 femtograms respectively. The two-step PCR protocol is recommended as a very sensitive and specific alternative protocol to Western blot assay for the detection of WSV. Copyright (C) 1999 Elsevier Science B.V.

Source or Periodical Title

Journal of Virological Methods

ISSN

1660934

Page

39-43

Document Type

Article

Subject

Detection, One-step, PCR, Two-step, WSV-DNA

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