Isolation, characterization and identification of bacteria from lahar

Issue Date

1997

Abstract

Samples of lahar and soil from Pampanga were collected and microbial counts were determined. In unaffected areas, the average colony-forming unit per milliliter (cfu) was 1.03 x 107 which is higher compared to that from the lahar-affected area (3.82 x 106 cfu/ml). Twenty-five of the 89 isolates were subjected to different morphological, cultural and physiological tests for their identification. Results of the tests showed that isolates from the surface of lahar-affected area belong to the following genera and species: Plesiomonas shigelloides (isolate L1-1), Acetobacter hansenii (L1-2), Acinetobacter calcoaceticus (L1-3), Proteus mirabilis (L1-4), Aeromonas caviae (L1-S), Bacillus coagulons (L1-6), Citrobacter freundii (L1-7), Kurthia gibsonii (L1-8), Micrococcus agilis (L1-9), and Bacillus subtilis (L1-26). For samples obtained from 5 cm depth in lahar-affected area, the following were identified: Micrococcus varians (isolate L2-1), Aeromonas caviae (L2-3), Staphylococcus (L2-4), Kurthia gibsonii (L2-5), Bacillus laterosporus (L2-6), Plesiomonas shigelloides (L2-7), Micrococcus agilis (isolates L2-8 and L2-11), Legionella morganii (L2-9) and Cellulomonas biazotea (L2-10). Different test results for isolates from 10 cm depth in lahar-affected area identified Micrococcus agilis (L3-1). In unaffected area, isolate UL1-1 obtained from the surface was identified to be Bacillus laterosporus. From 5 cm depth samples, different test results for isolate UL2-7 conformed with the characteristics of Bacillus laterosporus. Isolate UL3-3 and UL3-5 from 10 cm deep samples were identified to be Kurthia gibsonii based on their different test results.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Volume

80

Issue

1 & 2

Page

57-64

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Subject

Bacterial flora, Lahar

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