Protoplast formation, regeneration and fusion in Bacillus polymyxa

Abstract

Two-hour lysozyme treatment (1 mg/ml) of mid-logarithmic phase Bacillus polymyxa cells at 37°C resulted in 99% protoplasting efficiency. Among three typical regeneration media, HCP 1.5 gave the highest regeneration frequency of 1.8 × 10-2. Protoplast fusion between B. polymyxa B1103 Str+ His and B1104 Tyr- produced 39.6% recombinants of the total regenerants, Strr His+ Tyr- and Strr His- Tyr- being the major recombinant phenotypes. © 1991.

Source or Periodical Title

Journal of Fermentation and Bioengineering

ISSN

0922338X

Page

430-432

Document Type

Article

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