Transcriptome sequencing of Philippine beneficial insects

Issue Date

10-2017

Abstract

The parasitoid Comperiella and the stingless bee, Tetragonula sp. are known beneficial insects in the Philippines. Comperiella is able to parasitize cocolisap, a pest of coconut, and feeds on the internal structures, leading to eventual death of the pests. On the other hand, stingless bees are key pollinators in Philippine agriculture. This study aims to optimize a full-length protocol for the transcriptome sequencing of these beneficial insects using next generation sequencing (NGS) platforms, from RNA extraction to preparation of libraries and templates for NGS. Comperiella samples were obtained from San Roque, Sampaloc, Quezon, while stingless bee samples were collected from different provinces and cities in the Bicol Region. Total RNA was extracted from pupae and adult of Comperiella and adult for stingless bees. Extracted RNAs were then used for library preparation. Sequencing templates were then multiplexed, and paired-end sequencing was performed in a MiSeq sequencing platform. Sequencing results for Comperiella show a 6.36Gb read length, 20.84 M paired-end reads and 89.12% Q30 score, while for the stingless bee, a read length of 7.28Gb was generated, with 28.59 M paired-end reads, and a Q30 score of 89.64%. The transcriptome datasets obtained from these beneficial insects are currently undergoing bioinformatics analysis to completely provide the molecular toolboxes from which many genes of biological importance can be mined.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

31

Issue

2

Page

142

Document Type

Article

Frequency

semi-annually

Language

English

En – AGROVOC descriptors

COMPERIELLA; MELIPONA; APIDAE; INSECTA; USEFUL INSECTS; USEFUL ARTHROPODS; BIOLOGICAL CONTROL ARTHOPODS; POLLINATORS; RNA SEQUENCE; PARASITOIDS; PARASITES

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