Cloning and molecular characterization of chalcone synthase gene from mulberry (Morus alba L.)

Issue Date

8-2017

Abstract

Two members of the chalcone synthase (CHS) enzyme superfamily of type III polyketide syntheses (PKSs) are CHS and resveratrol synthase, which synthesize flavonoids and resveratrol respectively, and exhibit health and anti-fungal properties. This study aimed to clone, sequence and analyze partial CHS gene sequences from mulberr (M. alba L.) leaves using designed and published primers. CHS genes were isolated and cloned from mulberry genomic DNA through PCR-based methods using primers based on conserved regions of members of the CHS superfamily of type III PKSs. The 584-bp PCR amplicon generated two CHS clones having high sequence identity (>80 %) with CHS sequences from other plant sources. Phylogenetic analysis with other plant sources exhibited clustering of both sequences together with other angiosperm CHS sequences, specifically with dicots, which is consistent with mulberry classification. The CHS partial sequence corresponded to a 195 amino acid deduced protein, which exhibited several predicted conserved domains, including enzyme active site and dimer interface. Catalytic and conserved amino acid residues among CHS enzyme were present. Three dimensional homology modelling predicted a homodimeric protein with high homology to alfalfa CHS crystal structure.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

42

Issue

2

Page

1-9

Document Type

Article

Frequency

tri-quarterly

Physical Description

tables, pictures, dendrograms

Language

English

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