Molecular markers for improving selection of sugarcane varieties with down mildew resistance

Issue Date

4-2007

Abstract

DNA marker technology has provided new tools in various analyses ranging from phylogenetic analysis, development of high-density maps through PCR-based markers, DNA fingerprinting, cultivar identification, mapping and tagging genes of almost any trait possible. One of the techniques is the use of molecular markers by tagging genes that are linked to useful agronomic traits like disease resistance. This is done through the application of molecular marker-assisted selection (MAS). These markers can be used to identify disease resistant plants at the early stages of the breeding cycle, thereby reducing the costs, including labor and time, to produce new varieties of sugarcane. This study was conducted to identify reliable molecular markers for downy mildew resistance that can ultimately be incorporated in the sugarcane breeding program. A mapping population obtained from VMC86-550 X VMC88-354 consisting of 486 progenies were evaluated using 174 microsatellites (SSRs) primers to identify molecular markers for downy mildew resistance. The SSR primers were designed and developed based on the microsatellite-containing sequences of sugarcane by the International Consortium of Sugarcane Biotechnology (ICSB). Linkage map was constructed using JOINMAP statistical software based on the 147 molecular markers found to be polymorphic. Similarly, phenotypic data were analyzed to determine the association of downy mildew disease resistance to the marker using Fisher's exact test, Chi-square, and Bonferonni correction. A putative marker was identified to be associated with downy mildew resistance.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

32

Issue

1

Page

3-11

Document Type

Article

Frequency

tri-quarterly

Physical Description

tables, maps, pictures

Language

English

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