Molecular characterization of taro [Colocasia esculenta (L.) Schott] using microsatellite markers
Issue Date
12-2016
Abstract
Diversity of 46 taro [Colocasia esculenta (L.) Schott] accessions from the National Plant Genetic Resources Laboratory, Institute of Plant Breeding-Crop Science Cluster was analyzed using simple sequence repeats (SSR) to identify genetically different accessions that can be used as parents for taro varietal improvement. High quality DNA was isolated following modified Doyle and Doyle extraction protocol for taro and used for polymerase chain reaction (PCR). Fifteen sets of SSR primers based on taro, cassava and citrus sequences were used to amplify fragments. From these SSR primers, 10 resulted to band amplication. Polymorphic Information Content (PIC) was computed based on the bonding pattern produced. It ranged from 0.69-0.96 which indicated high genetic diversity. A dendrogram generated using NTSYS-pc formed 8 clusters using Jaccard's coefficient at 0.72. Among the clusters, there was no association between geographic origin and genotypes of germplasm resources observed. The results suggest that the accessions even within location are diverse, hence, can be used for taro breeding programs.
Source or Periodical Title
Philippine Journal of Crop Science
ISSN
0115-463X
Volume
41
Issue
3
Page
65-73
Document Type
Article
Physical Description
illustrations; tables
Language
English
Subject
Taro; Microsatellites; Plant breeding
Recommended Citation
Rasco, J.L. S.; Mendoza, M.R. R.; Abustan, M.A. M.; and Lalusin, A. G., "Molecular characterization of taro [Colocasia esculenta (L.) Schott] using microsatellite markers" (2016). Journal Article. 4523.
https://www.ukdr.uplb.edu.ph/journal-articles/4523
En – AGROVOC descriptors
COLOCASIA ESCULENTA; TARO; DNA; GENETIC MARKERS; GENOTYPES; GERMPLASM; GENETIC VARIATION
