Molecular characterization of taro [Colocasia esculenta (L.) Schott] using microsatellite markers

Issue Date

12-2016

Abstract

Diversity of 46 taro [Colocasia esculenta (L.) Schott] accessions from the National Plant Genetic Resources Laboratory, Institute of Plant Breeding-Crop Science Cluster was analyzed using simple sequence repeats (SSR) to identify genetically different accessions that can be used as parents for taro varietal improvement. High quality DNA was isolated following modified Doyle and Doyle extraction protocol for taro and used for polymerase chain reaction (PCR). Fifteen sets of SSR primers based on taro, cassava and citrus sequence were used to amplify fragments. From these SSR primers, 10 resulted to band amplification. Polymorphic Information Content (PIC) was computed based on the banding pattern produced. It ranged from 0.69-0.96 which indicated high genetic diversity. A dendrogram generated using NTSYS-pc formed 8 clusters using Jaccard's coefficient at 0.72. Among the clusters, there was no association between geographic origin and genotypes of germplasm resources observed. The results suggest that the accessions even within location are diverse, hence, can be used for taro breeding programs.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

41

Issue

3

Page

65-73

Document Type

Article

Frequency

tri-quarterly

Physical Description

pictures, tables, dendrograms

Language

English

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