Taxonomic identification and molecular characterization of Toxorhynchites species (Diptera: Culicidae: Toxorhynchitinae) from Mt. Makiling, Laguna, Philippines

Issue Date

10-2014

Abstract

Mosquitoes belonging to the genus Toxorhynchites have shown great potential for biological control of some medically important pests. Specimens were collected from August 2013 to March 2014 by examination of possible breeding sites such as tree holes, cut bamboo internodes, leaf axils, and artificial containers complemented by ten black oviposition traps in Mt. Makiling, Laguna. Specimens used for identification were laboratory-reared adults that emerged from field-collected immature fed with Aedes aegypti (SK use) larvae. Using dichotomous keys for oriental species, one set of specimens were initially identified as Toxorhynchites splendens (Weidemann). A confirmatory step using sequences of the cytochrome c oxidose I (COI) gene run on a Basic Local Alignment Search Tool (BLAST) revealed a 97% similarity with Tx. splendens found in genebank. The ultrastructure and the taxonomic characters used to distinguish this species from the Philippines are presented together with the DNA barcode.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

28

Issue

2

Page

208

Document Type

Article

Frequency

semi-annually

Language

English

En – AGROVOC descriptors

TOXORHYNCHITES; ANIMALS; SPECIES; TAXA; FOREST RESERVES; TAXONOMY; BIOLOGICAL CONTROL; IDENTIFICATION; GEOGRAPHY; PHILIPPINES

This document is currently not available here.

Share

COinS