New genotypes of aflatoxigenic fungi from Egypt and the Philippines

Issue Date

5-2020

Abstract

Aflatoxins (AFs), mainly produced by Aspergillus section Flavi, are the major natural toxins of crops and commodities in hot climatic geographic regions. These toxins are considered as type A carcinogens. One hundred and sixty single spore isolates of A. section Flavi were isolated from two different geographical places, Egypt and the Philippines. A quarter (26.5%) of the isolates was able to produce AFs. Four chemotypes of aflatoxin-producing fungi were obtained. Surprisingly, all aflatoxin-producing A. nomius isolates produced higher amounts (2400-40400 ng ml-1) of total AFs (AFB1, AFB2, AFG1 and AFG2) than the toxigenic A. flavus isolates (ml-1). All isolates producing AFs gave PCR products with the ver-1/ver-2 and ordAF/ordAR primers, which amplify ver-1 and ordA genes in the aflatoxin biosynthetic pathway. Based on PCR products of ver-1 gene, new genotypes of aflatoxigenic fungi were found which revealed the variability of AFs production between different isolates depending on the region of the isolation.

Source or Periodical Title

Current Research in Environmental and Applied Mycology

ISSN

2229-2225

Volume

10

Issue

1

Page

142-155

Document Type

Article

Physical Description

illustration, tables, references

Language

English

Subject

Aflatoxin, Aspergillus section flavi, Fungi, PCR

Digital Copy

Yes

En – AGROVOC descriptors

AFLATOXIN; ASPERGILLUS SECTION FLAVI; FUNGI; PCR

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