Frequency of alleles conferring major resistance to bacillus thuringiensis toxin upon a philippine population of the stripped stemborer, Chilo Suppressalis
Issue Date
10-2002
Abstract
The F2 screen methodology entails sib-mating within isofemale lines todetect any resistance allele that was intially present in the fertilized eggs of field collected females. We applied the F2 screen methodology to the stripped stemborer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae), using IR72 rice plants transformed with a cry1Ab/cry1Ac hybrid gene. When all 151 isofemale lines were included in the analysis, the frequency of alleles encoding major resistance to the Cry1Ab/Cry1Ac toxin was <1.6 X 10-3 with a 96% detection probability. The upper 95% Credibility Intervals of the frequency of such allele was 4.8 X 10-3. By analyzing the subsets of data consisting of 151 isofemale lines that produced = 1, 2, 3, 4, and 5 F, families per isofemale line, a conservative estimate of allele frequency would rely on data with = 3 F1 families per isofemale line with a high value (99%) of detection probability. Our results suggest that the frequency of allele conferring resistance to the Cry1Ab/Cry1Ac hybrid toxin in the population of C. suppressalis sampled approaches 10-3, which had been suggested as the maximum initial resistance allele frequency that would enable the high does/refuge resistance management strategy to be successfully applied.
Source or Periodical Title
Philippine Entomologist
ISSN
0048-3753
Volume
16
Issue
2
Page
156
Document Type
Article
Frequency
semi-annually
Language
English
Recommended Citation
Cuong, N. L.; Andow, D. A.; Adalla, C. B.; Cohen, M. B.; and Leeb, D., "Frequency of alleles conferring major resistance to bacillus thuringiensis toxin upon a philippine population of the stripped stemborer, Chilo Suppressalis" (2002). Journal Article. 5806.
https://www.ukdr.uplb.edu.ph/journal-articles/5806
En – AGROVOC descriptors
CHILO SUPPRESSALIS; LEPIDOPTERA; INSECTA; PLANT PESTS; BACILLUS THURINGIENSIS; PHILIPPINES