The use of dark field photomicrography in the assessment of sperm motility in buffaloes, bulls, and bucks

Issue Date

7-1999

Abstract

The adoption of dark field photomicrography as an objective measure of sperm motility is herein presented. Drops of buffalo, bull and buck semen (fresh or frozen) were placed on prewarmed glass slides, covered with coverslips and photographed (1- and 2-second exposure time, low and high power magnification) to identify the tracks of motile spermatozoa.

Only 1% to 2% difference between percentage motility counts was obtained by using different magnifications and exposure times. Dark field photomicrography could be applied to both fresh and frozen semen. An objective, rapid, reproducible and accurate method, it is applicable and effective in the assessment of sperm motility in buffaloes, bulls and bucks.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

82

Issue

3

Page

318-326

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

illustrations; references

Language

English

En – AGROVOC descriptors

SPERMATOZOA; SEMEN; LOCOMOTION; WATER BUFFALOES; BULLS; BILLYGOATS; PHOTOMICROGRAPHY; VISUAL INSPECTION

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