Molecular detection of Cryptosporidium from animal hosts in the Philippines

Issue Date

12-2008

Abstract

Cryptosporidium spp. are organisms of public health importance and are known to infect numerousanimal hosts. In the Philippines, information on Cryptosporidium is limited. In this study, stool samplesfrom calves, goats, pigs, chickens and monkeys were collected and a fragment of the Cryptosporidiumheat-shock protein gene was amplified by polymerase chain reaction (PCR) to detect the infection.Pigs showed the highest rate of infection (34.29%) followed by calves (20.41%) and chickens (5.66%).This is the first record of Cryptosporidium infection in pigs and chickens in the Philippines. In addition,a fragment of the Cryptosporidium SSU rRNA gene was amplified and sequenced to genotype theisolates from the stool of calves. Cryptosporidium parvum and C. canis were identified. This is the firstreport of genotyping of Cryptosporidium spp. in the Philippines.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

91

Issue

4

Page

473--477

Document Type

Article

Frequency

quarterly

Physical Description

illustration ; tables ; references

Language

English

Subject

calf, chicken, Cryptosporidium, genotype, heat shock protein gene, PCR, Philippines, pig, SSU rRNA gene

En – AGROVOC descriptors

CRYPTOSPORIDIUM; PROTOZOAL INFECTIONS; PARASITES; GASTROINTESTINAL DISEASES; ANIMAL DISEASES; NUCLEOTIDE SEQUENCE; DETECTION

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