Maize pollen mutation by low energy ion beams

Issue Date

12-2008

Abstract

A mensuration method to evaluate pollen viability with low energy in maize was established in this study. Several challenges experienced during ion implantation pollen mutation research were overcome, including pollen loss from run-off during vacuum pumping, and high pollen mortality rates. The data showed that a half lethal dose of 1×1014 N+ cm-2 at low-energy 30 keV was most effective for treating maize pollen. Three elite inbred lines were treated in this study. Some mutants such as white anther color, red seed capsules, lethal seed and albino seedling mutants were observed in the M0 and M1 populations. The results demonstrated that the pollen treatment with low-energy ion beam was an effectual method in maize mutant breeding.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

91

Issue

4

Page

395-400

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

illustrations; tables; references

Language

English

Subject

maize, pollen, low energy ion beams, mutation, radiation

En – AGROVOC descriptors

MAIZE; POLLEN; IONS; MUTATION; POLLEN GERMINATION; VIABILITY

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