An efficient method for the simultaneous recovery of DNA and RNA from soil microbes in clayish and acidic soils

Issue Date

6-2008

Abstract

Recently, molecular biology techniques have been widely used to study the structure diversity and function of soil microbial communities. The basis of these techniques is the extraction of sufficient and high purity DNA or RNA from soil samples. Because of a high clay content and low pH in the soils of subtropical China, it is difficult to extract DNA or RNA from the soils from this region. In this study, by combining the pre-lysis washing procedure and the direct extraction method, DNA and RNA were simultaneously recovered from two typical soils in subtropical China, and the recovery efficiency was estimated. Analysis of the extracted DNA or RNA with enzyme digestion, polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR) showed that the purity of the nucleic acid was suitable for follow-up procedures. This provides a feasible way to further study microbial activities and function in the soils of subtropical China and similar soils elsewhere.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

91

Issue

2

Page

143-149

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

illustrations, tables, references

Language

English

Subject

enzyme digestion, nucleic acids recovery, polymerase chain reaction, recovery efficiency, washing procedure

En – AGROVOC descriptors

SOIL MICROORGANISMS; CLAY SOILS; ACID SOILS; DNA; PCR; RNA; RECUPERATION; MOLECULAR BIOLOGY

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