Antimicrobial resistance profile of Escherichia coli isolated from pork in public markets in Laguna, Philippines

Issue Date

7-2021

Abstract

The emergence of antimicrobial resistance among Escherichia coli isolates is a serious problem globally complicated by its dissemination through the food chain. Since Filipinos consume pork the most, the aim of this study is to isolate and subsequently, characterize the antimicrobial resistance profile of E. coli derived from pork in the Philippines. A total of 27 E. coli isolates were recovered from pork purchased from 19 public markets in Laguna, Philippines. Standard selective isolation and biochemical identification were employed whereas confirmation was performed using RapID™ ONE kit. Minimum inhibitory concentrations (MIC) were determined using the Clinical and Laboratory Standards Institute (CLSI) broth microdilution guidelines. Out of the 19 public markets, 14 (63%) were positive for the presence of E. coli in pork chops. Resistance was most frequently observed in tetracycline (96%) and doxycycline (93%), followed by cephalothin (89%), trimethoprim (89%), ampicillin (78%), chloramphenicol (70%), and ciprofloxacin (48%); whereas fewer resistant isolates were detected against kanamycin (4%) and gentamicin (4%). All isolates were resistant to at least three antimicrobials indicating a 100% multidrug-resistant bacterial population. The results suggest that pork is an important reservoir of antimicrobial-resistant E. coli exposing the public to potential health hazards in the food chain.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

0031-7705

Volume

58

Issue

2

Page

201-202

Document Type

Article

Frequency

semi-annually

Physical Description

tables

Language

English

En – AGROVOC descriptors

PORK; ANTIMICROBIAL RESISTANCE; ESCHERICHIA COLI; ANTIBIOTICS; FOOD HYGIENE; PHILIPPINES

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