The potential bacteriocinogenic activity of putative bacteriocins of Escherichia coli from bat guano against enterohemorrhagic E. coli strains

Issue Date

7-2021

Abstract

This study investigated the potential bacteriocinogenic property of E. coli isolates against two EHEC strains using the Deferred Growth Inhibition, Spot-on-Lawn, and Bacteriocin Sensitivity Assays, in the search for new antimicrobials against some pathogenic E. coli serotypes. Among 62 E. coli strains evaluated (sourced from native and commercial chickens, and from bat guano through UPLB-MNH), only MNH strains 1647, 1648, 1651, and 1652 showed antagonistic activity against the EHEC strains in the Deferred Assay. In the Spot-on-Lawn Assay, none of the preparations from the four isolates heated at 90'C for 10 minutes inhibited the test strains suggesting heat sensitivity of the inhibitory substance, similar to many bacteriocins. In the Bacteriocin Sensitivity Assay, only MNH 1647 and 1651 inhibited another E. coli strain (DH5a) on LB Agar, with or without the stress inducer Mitomycin C. Significantly bigger zones of inhibition relative to the controls were observed with sonicated cell and cell-free supernatant preparations of the isolates treated with Mitomycin C. This study proved that bat guano E. coli could be potential sources of putative bacteriocins/bacteriocin-like substances against some EHEC strains and that Mitomycin C seemed to enhance the putative bacteriocinogenic activity of preparations from the bat guano isolates.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

0031-7705

Volume

58

Issue

2

Page

167-181

Document Type

Article

Frequency

semi-annually

Physical Description

illustrations; tables, graphs

Language

English

En – AGROVOC descriptors

CHIROPTERA; MAMMALIA; ESCHERICHIA COLI; BACTERIOCINS; MITOMYCIN

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