Serological and molecular detection of newcastle disease virus in cattle egrets (Bubulcus ibis) in Bulacan, Philippines

Abstract

© 2019, University of the Philippines at Los Banos. All rights reserved. Cattle egrets (Bubulcus ibis) are suspected reservoirs of Newcastle disease for they are closely associated with migratory fowl and habitually interact with poultry populations for food and water. Nineteen cattle egrets of various ages and sexes, from three different municipalities in Bulacan, Philippines, were examined for antibodies against ND and its viral antigen through hemagglutination inhinbition (HI) test and nested reverse-transcriptase polymerase chain reaction (nested RTPCR). All egrets were apparently healthy upon physical examination, apart from two that had soiled vents, and two which died during transport. Weights of these birds ranged from 0.6 to 1.2 kg. Oropharyngeal, choanal, tracheal, cloacal swabs, as well as tissue samples were collected and subjected to both HI test and NRT-PCR and HI test. Results showed 100% and 88.24% prevalence of the NDV and antibodies, respectively. This first report on the occurrence of NDV and its antibodies in apparently healthy cattle egrets suggests that there was an active ongoing infection and possible exposure to either field or vaccine viruses during the time of collection. This study shows that cattle egrets may serve as potential reservoirs for the virus.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

317705

Page

2016-10-01

Document Type

Article

Subject

Cattle egrets, Hemagglutination inhibition, Nested RT-PCR, Newcastle disease

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