Effect of dietary supplementation with β-mannanase on the small intestinal morphology and intraepithelial lymphocytes of broilers
Abstract
© 2019, University of the Philippines at Los Banos. All rights reserved. β-mannan is identified as one of the anti-nutritional factors (ANF) present in diets of broilers resulting to reduced absorption of nutrients and impaired immunologic functions of the animal. This study was conducted to determine the effects of β-mannanase supplementation on small intestinal morphology and number and localization of intraepithelial lymphocytes (IEL) of broilers fed with corn-soybean meal-based diets. Four soybean meal-based diets with varying doses of the enzyme (0 ppm, 40 ppm from competitor brand, 40 ppm, and 80 ppm) were used. Broilers were fed mash booster (d 0 to 10), starter (d 11 to 21), and finisher diets (d 22 to 35). Intestinal samples were collected and analyzed via light microscopy. Analysis with Tukey’s Post Hoc Test revealed that mucosal thickness throughout the small intestinal tract increased significantly (p<0.05), as well as duodenal and jejunal villous height and a decrease in jejunal crypt depth at as low as 40 ppm inclusion of β-mannanase. Jejunal IEL also decreased in numbers with increasing amount of enzyme in the diet. This study has shown positive changes elicited by β-mannanase supplementation on small intestinal morphology and immunity of broilers.
Source or Periodical Title
Philippine Journal of Veterinary Medicine
ISSN
317705
Page
28-35
Document Type
Article
Subject
Anti-nutritional factors, Intraepithelial lymphocytes, Mucosal immunity, Small intestinal morphology, β-mannanase
Recommended Citation
Lagahid, Kriselle Febby S. and Dela Cruz, Joseph F., "Effect of dietary supplementation with β-mannanase on the small intestinal morphology and intraepithelial lymphocytes of broilers" (2021). Journal Article. 815.
https://www.ukdr.uplb.edu.ph/journal-articles/815