Effect of dietary supplementation with β-mannanase on the small intestinal morphology and intraepithelial lymphocytes of broilers

Abstract

© 2019, University of the Philippines at Los Banos. All rights reserved. β-mannan is identified as one of the anti-nutritional factors (ANF) present in diets of broilers resulting to reduced absorption of nutrients and impaired immunologic functions of the animal. This study was conducted to determine the effects of β-mannanase supplementation on small intestinal morphology and number and localization of intraepithelial lymphocytes (IEL) of broilers fed with corn-soybean meal-based diets. Four soybean meal-based diets with varying doses of the enzyme (0 ppm, 40 ppm from competitor brand, 40 ppm, and 80 ppm) were used. Broilers were fed mash booster (d 0 to 10), starter (d 11 to 21), and finisher diets (d 22 to 35). Intestinal samples were collected and analyzed via light microscopy. Analysis with Tukey’s Post Hoc Test revealed that mucosal thickness throughout the small intestinal tract increased significantly (p<0.05), as well as duodenal and jejunal villous height and a decrease in jejunal crypt depth at as low as 40 ppm inclusion of β-mannanase. Jejunal IEL also decreased in numbers with increasing amount of enzyme in the diet. This study has shown positive changes elicited by β-mannanase supplementation on small intestinal morphology and immunity of broilers.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

317705

Page

28-35

Document Type

Article

Subject

Anti-nutritional factors, Intraepithelial lymphocytes, Mucosal immunity, Small intestinal morphology, β-mannanase

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