Draft genome sequences of ralstonia solanacearum isolated from banana and tomato in the Philippines

Abstract

© 2019, Department of Science and Technology. All rights reserved. Ralstonia solanacearum causes bacterial wilt of several plant species, including banana and tomato. With limited options for control, understanding the molecular mechanism of pathogenicity is warranted. Herein, we report the draft genome sequences of two R. solanacearum isolates from the Philippines infecting banana and tomato. R. solanacearum 10314 was isolated from banana while R. solanacearum 10154 was isolated from tomato. Pathogenicity tests indicated that 10314 can infect both banana and tomato while 10154 can only infect tomato. In an effort to investigate the molecular basis of virulence and differential host-specificity of the isolates, whole genome sequencing was performed using the Ion Torrent Proton platform. Draft assemblies were generated using three assemblers, and the quality was evaluated using assembly metrics. Standard genome annotation was performed allowing for identification of important virulence-and host-specificity-related genes for the bacterial isolates, which provided clues underlying their differential capacity to infect banana and tomato. The availability of these data in public repositories will complement the existing data from several R. solanacearum strains, including those isolated from the Philippines; thus, it can provide essential platforms for studying R. solanacearum pathogenicity and help in the control of bacterial wilt.

Source or Periodical Title

Philippine Journal of Science

ISSN

317683

Page

115-132

Document Type

Article

Subject

Host specificity, Pathogenicity, Ralstonia solanacearum, Virulence genes, Whole-genome sequencing

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