Screening of resistant starch in indigenous Philippine root crops as an index of prebiotic potential
Date
5-2015
Abstract
Prebiotics are non-digestible compounds that selectively favored the growth of beneficial microorganisms in the gastrointestinal tract. Different root crops were processed into flour prior to characterization as prebiotic. This study determined the resistant starch and total dietary fiber content of two varieties of Ubi (Magnolia and Hinaligi), four varieties of sweet potato (purple yellow), Taiwan (orange) and Super Bureau (white), cassava (yellow), and arrow root as an indicator of its prebiotic potential. The highest resistant starch content was observed in the ubi variety Hinaligi at 14.40 g/100 g and the lowest was obtained in arrow root at 1.88 g/100g. Total dietary fiber content, on the other hand, was highest at 27.59% (w/w) in sweet potato Taiwan variety and lowest in cassava at 5.51 g/100 g. Three species of the genus Lactobacillus namely: L. acidophilus, L. plantarum and L. casei, were chosen as representative of the beneficial microflora of the gut. Escherichia coli, on the other hand, was chosen to represent in the non-beneficial microorganisms in the gastrointestinal tract. Evaluation of the prebiotic activity score was done through a monoculture in vitro method in a basal medium devoid of carbon source and supplemented with the different root crop flour. Cell growth and lowering of pH of the medium after incubation was used as indices of the ability of the test microorganisms to ferment the different root crop flours. Highest prebiotic activity score (0.83) was exhibited by Lactobacillus acidophilus when grown in a culture medium supplemented with 1% sweet potato Super Bureau flour. The lowest prebiotic activity score (0.22) was shown in cassava flour by L. plantarum. In general, the flour with the highest resistant starch and total dietary flour content exhibited promising prebiotic activity.
Language
English
Document Type
Article
Recommended citation
Mopera, L. E.; Del Rosario, O. M.; and Algar, A.F. C., "Screening of resistant starch in indigenous Philippine root crops as an index of prebiotic potential" (2015). Report. 1051.
https://www.ukdr.uplb.edu.ph/reports/1051
Pages /Collation
55 leaves
En – AGROVOC descriptors
SWEET POTATOES; SPECIES; YAMS; ARROWROOT; NONCEREAL FLOURS; FOOD COMPOSITION; DIETARY FIBRES; INDIGENOUS ORGANISM; LACTOBACILLUS ACIDOPHILUS; LACTOBACILLUS CASEI; LACTOBACILLUS PLANTARUM; ESCHERICHIA COLI; PHILIPPINES