Ensuring the availability of high quality microbial strains for biofertilizers, biostimulants and biopesticides production through the development of molecular markers, polyphasic identification, long-tem preservation and enhanced bio-banking system (project 8)

Date

2015

Abstract

A total of 72 bacterial and/or fungal isolates from the 8 projects of the BFSP program were characterized and identified by polyphasic (phenotypic, molecular and phylogenetic) approach. A consensus of these characteristics was done to properly identify the strains. Sixty two of these 72 are newly-isolated microorganisms by the different projects of the program, the 10 are existing strains deposited at the Philippine National Collection of Microorganisms (PNCM). All 72 isolates were accessioned at the PNCM and are preserved using at least 2 different methods. Bacterial and yeast isolates were L-dried and preserved at ultra-low temperature(-80 deg C) in silica beads using glycerol; mold isolates were preserved by mineral oil overlay and ultra-low temperature (-80 deg C) with glycerol; and actinomycetes were preserved by L-drying, mineral oil overlay and sterile soil as backup. Forty-six bacteria, 10 yeast, 5 mold and 11 actinomycete isolates had been preserved. For ease of detection in the presence of closely-related strains in the field, DNA fingerprinting using ERIC, BOX and REP-PCR was done for 16 isolates (two most promising strains from each project).

Language

English

Document Type

Article

Pages /Collation

203 leaves

En – AGROVOC descriptors

MICROORGANISMS; BIOFERTILIZERS; BIOPESTICIDES; GENETIC MARKERS; PRESERVATION; DNA; ACTINOMYCETALES; PCR

This document is currently not available here.

Share

COinS