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The Philippine Agricultural Scientist

Publication Date

3-31-2021

Abstract

We studied the inactivation kinetics of Cronobacter sakazakii (CS) in infant formula subjected to radio frequency dielectric heating (RF) alone or sequential RF-UVC irradiation for 30 min. We used one linear model and two nonlinear models to fit the data and compared the results. Our findings revealed that CS was more susceptible to the combined treatment than to RF alone at the same temperature and that inactivation rate increased with increasing temperature. The sequential RF (90°C, 5 min) - UVC (25 min) treatment was the most effective at inactivating CS. The survival curves of CS were non-linear. The modified log-logistic model (R2 = 0.9783–0.9904, RMSE = 0.02–0.05, Af =1.04–1.07, Bf = 0.99–1.03) generated a better fit than the Weibull (R2 = 0.9111–0.9863, RMSE = 0.02–0.10, Af = 1.04–1.16, Bf =1.00–1.09) or first-order kinetics models (R2 = 0.6514–0.7937, RMSE = 0.07–0.30, Af = 1.12–1.77, Bf = 0.93–1.38). Overall, the survival curves of CS in infant formula subjected to RF alone or sequential RF - UV light were nonlinear. The modified log-logistic model may be a useful tool to describe the inactivation patterns for CS in infant formula, and our results also demonstrated the high color stability of samples after sequential RF - UVC treatments.

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