Effect of Vitamin C and Probiotics on Broilers Performance and Claudin-2 (CLDN-2) Expression Under Heat Stress [RESEARCH NOTE]

Authors

Mohammad Chamani, Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, IranFollow
Mohammad Chamani, Department of Animal Science, Science and Research Branch, Islamic Azad University, , Tehran, IranFollow
Farhad Foroudi, Department of Animal Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, IranFollow
Ali Asghar Sadeghi, Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, IranFollow
Mehdi Aminafshar, Department of Animal Science, Science and Research Branch, Islamic Azad University, , Tehran, IranFollow

Publication Date

6-1-2023

Abstract

This study was conducted to investigate the effect of vitamin C and probiotic which consists of five species of beneficial bacteria and two species of fungi on performance, blood parameters, and intestinal CLDN-2 gene expression of broilers during heat stress (HS). A total of 400 broilers were randomly divided into five groups, each of which received a different treatment. The first group was considered a negative control and did not experience any HS (C^-); the second group was considered a positive control (C⁺) and was exposed to HS; the third group received only vitamin C (250 mg/L in drinking water); the fourth group received only the recommended dose of probiotics; and the fifth group received a combination of both vitamin C and probiotics (250 mg/L in drinking water and recommended dose, respectively). The broiler’s performance, blood parameters, and intestinal CLDN-2 gene expression were assessed. The results showed that the administration of probiotics and vitamin C increased the broiler's feed intake and decreased the feed conversion ratio (p < 0.05). Heat stress increased the broiler's blood total protein, glucose, cholesterol, triglyceride, and liver enzyme concentration (p < 0.05). While the supplementation of vitamin C and probiotics decreased these parameters during HS (p < 0.05). Vitamin C administration reduced the intestinal CLDN-2 gene expression in broilers, during HS (p < 0.05). Heat stress negatively affected the broiler's performance and increased the mortality rate and intestinal CLDN-2 gene expression, whereas, the supplementation of vitamin C decreased the deleterious effect of HS in broilers.

Recommended Citation

Chamani, Mohammad; Chamani, Mohammad; Foroudi, Farhad; Sadeghi, Ali Asghar; and Aminafshar, Mehdi (2023) "Effect of Vitamin C and Probiotics on Broilers Performance and Claudin-2 (CLDN-2) Expression Under Heat Stress [RESEARCH NOTE]," The Philippine Agricultural Scientist: Vol. 106: No. 2, Article 2.
Available at: https://www.ukdr.uplb.edu.ph/pas/vol106/iss2/2