Determining the efficacy of extracted propolis as a fungicide against Ascosphaera apis and Aspergillus niger

Date

6-2024

Academic Strand

Science, Technology, Engineering, and Mathematics (STEM) Strand

Adviser

Aprhodite M. Macale

Principal

Buela, Mabel S.

Abstract

Propolis is a product that bees produce by collecting various materials in the surroundings and is known to have antifungal properties. Through time the number of fungi that causes death to bee colonies such as Ascosphaera apis and Aspergillus niger has increased causing a decrease in the bee population and problems for the beekeepers. Hence, studies to identify antifungal agents to fight against these fungi have been conducted in various countries. However, the possible utilization of propolis as antifungal agent against the fungi that affect them has not been studied. Conversely, this study aims to determine whether the extracted propolis can be used to battle against the two previously mentioned fungi. The specific objectives of this study are to extract propolis through maceration using ethanol as the solvent, to chemically characterize the sample through gas chromatography/mass spectrometer (GCMS), and to test its antifungal property against Ascosphaera apis and Aspergillhus niger. The extraction was done using a shaker where the crude propolis was macerated. Afterwards, it was filtered, underwent rotary evaporation and the solid extract underwent GCMS where its chemical composition was determined. From this, the agar well diffusion method was used to determine its inhibitory activity recorded with the inhibition zones. The results of the experiment showed that the 1:9 concentration of extracted propolis macerated at a l:4 ethanol concentration for 6 hours has no significant antifungal activity against Aspergillus niger and no concludable antifungal activity against Ascosphaera apis. It is recommended to optimize the extraction and antifungal testing of stingless bee propolis. to further explore its antifungal properties.

Language

English

LC Subject

Capstone

Location

UP Rural High School

Document Type

Capstone

This document is currently not available here.

Share

COinS