Comparative electrophoretic analysis of peroxidase and glutamate oxaloacetate transaminase in three species of Nicotiana

Date

3-1988

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Azucena L. Carpena

Abstract

The ontogeny, organ and species especificity of peroxidase (PX) (B. C. 1.00.0.7) and glutamate oxaloacetate transaminase (GOT) (E>C> 2.6.0.1) isozyme patterns in Nicotiana were comparatively analyzed by starch gel electrophoresis. Peroxidase zymograms were characterized by five sites of enzyme activity (PX-1 to PX-5) each representing a group of adjacent isoperoxidases. Zymograms produced from young, mature, and senescent leaves showed a total of 5, 11 nad 16 isoperoxidases, respectively, thereby previous studies indicating a pattern of increasing of isozymes with leaf age. Particular bands at PX-1 had always been present in high concentrations regardless of leaf development stages. Zymogram obtained using the remote, flowers, and stems of three Nicotiana species revealed both organ and species-specific patterns. Relative position of each isoperoxidase group shifted more dramatically among of different species than among organs of a single species. Two GOT isozymes designated as GOT-1 and GOT-2, were detected from young leaf-samples. Only GOT-1 was observed when mature and senescent leaves were used. GOT activity was identical for the roots, flowers, and stems of three Nicotiana species, showing no indication of any organ and species pattern. The probable factors that may account for the observed ontogenetic, organ, and species-associated isozyme expressions in the enzyme systems were discussed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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