The role of lectin in the expression of bacterial wilt resistance in tomato (Lycopersicon esculentum L.)

Date

10-1998

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Experiments were conducted to investigate lectin and its behavior in bacterial wilt resistance in tomato (Lycopersicon esculemum) lines LIPL.Fm95-1 1 (susceptible), 94-304 (resistant), and their F, and F, progenies upon inoculation with virulent Ralsionia solanacearum. Wilt score was monitored and lectin content was quantified using enzyme-linked immunosorbent assay (ELISA). The ELISA for lectin determination which was developed in this study was found to be fast and sensitive for monitoring low lectin levels in small plant parts and in handling a large number of samples. its detection limit was about 0.2 ng lectin in sample. Lectin level in tomato plants was generally found to increase in response to bacterial inoculation. A comparison of lectin and bacterial densities against time prior to wilting indicated lectin-pathogen interaction. The trend of changes in lectin content increase in resistant plants was quadratic with a maxima on day 6 post-inoculation while it was quartic for the susceptible line with peak values at day 3 and 9 post-inoculation. Bacterial count and lectin concentration did not vary significantly among various stem sections of both resistant and .susceptible plants upon inoculation. Resistant plants were found to inhibit bacterial multiplication. with susceptibility of tomato cultivars in this study, probably followed a digenic mode of inheritance with complimentary gene action. Two dominant genes may be required for the expression of bacterial wilt susceptibility. Inheritance of lectin is possibly influenced by at least 2 genes with gene linkages. Changes in lectin concentration before wilting indicated the involvement of other defense mechanism(s) or factors(s) to prevent bacterial proliferation, since lectin alone could not fulfill this role. A new proposal of lectin-bacteria interaction was made to explain the resistance phenomenon wherein lectin acts as a major mediating element in tomato wilt resistance.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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