Assessment of genetic relationships in selected IPB- developed Maize (Zea mays) lines using RAPD markers

Date

10-1997

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

Random amplified polymorphic DNA (RAPD) markers were used to establish the identity of eight IPB-developed maize inbred lines and four hybrids, to detect hybridity and to evaluate their genetic relationships. Conditions for RAPD assays in maize were optimized. The homogeneity of the samples was determined in 5 plants each from two inbreds (Pi 17 and Pi 21) and 4 plants from each of the two hybrids (IPB 929 and IPB 949) using primers OPV-18 and OPZ-19. Twenty Operon primers were screened for their ability to detect polymorphism among the 12 lines. Among the 20 primers, eight primers yielded at least one amplification product. Five of these primers (OPE-08, OPE-15, OPE-17, OPX-20 and OPZ-16) gave 19 reproducible RAPD markers, and 17 of which were polymorphic. The estimated genetic relationships between the corn lines agreed to a limited extent with the expected results based on pedigree data.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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