Comparison of methods for determination of crude fat in coconuts (Cocos nucifera L.)

Date

3-1991

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Lilia S. P. Madamba

Abstract

Two sets of samples for coconut meat and copra were extracted using 4 methods: (1) Goldfisch extraction by petroleum ether; (2) Goldfisch extraction by petroleum ether:diethyl ether:ethanol (1:1:1 in v/v/v); (3) Goldfisch extraction by chloroform:methanol (2:1 in v/v); and (4) combined acid hydrolysis with sequential solvent extraction with ethanol, diethyl ether and petroleum ether. Comparison of methods showed that generally Method 3 (CHC1 :Me0H) is the most efficient in terms of % oil 3 extracted for both coconut meat and copra. Method 1 (PE) and Method 2 (PE:DE:EtOH) yielded results that are in some case comparable while varying results for the two sets of samples were observed for Method 4 (acid). Silicic acid column chromatography was used to fractionate the lipid samples into their neutral, glycolipid and phospholipid fractions. Results showed that for % neutral fractions, Method 2 (PE:DE:EtOH) gave the highest yield for coconut meat while Method 4 (acid) gave the highest for copra. On the other hand, Method 3 (CHC1 tMe0H) gave the highest glycolipid and phospholipid . 3 fractions for both samples.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS