Preliminary study of phenolic compounds present in stingless bee (Trigona biroi Friese) and honey bee (Apis mellifera Linnaeus) propolis using tandem liquid chromatography/mass spectrometry

Date

4-2011

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Jose Rene L. Micor

Co-adviser

Cleofas R. Cervancia

Abstract

The possible phenolic compounds present in Apis mellifera Linnaeus and 7'rigona biroi Fricse propolis were identified using tandem liquid chromatography mass spectrometry and the total phenolic content of both propolis samples were determined using Folin-Ciocalteau assay. This study was able to identify some phenolic compounds present in A.mellifera and Tbiroi propolis. There were 14 identified phenolic compounds present in A.mellikra propolis and 8 identified phenolic compounds present in T.birai propolis. Flavonoids are the major group of phenolic compounds present in both samples. The results of this study strongly suggest the presence of luteolin-5-methyl-ether, pinobanksin-5, 7-dimethylether and Artepilin C in both A.mellyera and T.biroi propolis. The total phenolic content of methanolic and ethanolic extracts were determined. Results showed that the methanolic extracts of A.mellifera and T.biroi have only small difference in their total phenolic content. The Tbiroi extract contained 2.44 ± 3.30x10' mg/g dry extract and A.mellifera propolis contained 2.49 ± 5.94 x10-3 mg/g dry extract. For the ethanolic extract, T.biroi propolis contained 5.38 ± 3.09x104 mg/g dry extract which is higher compared to A.mellyera propolis which contained 2.54 ± 3.17x104 mg/g dry extract. The total phenolic content of ethanolic extracts was found to be higher than the methanolic extracts for both A.mellifera and T biroi.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

"LG 993.5 2011 C4 /L43"

Document Type

Thesis

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