Preliminary study of phenolic compounds present in stingless bee (Trigona biroi Friese) and honey bee (Apis mellifera Linnaeus) propolis using tandem liquid chromatography/mass spectrometry
Date
4-2011
Degree
Bachelor of Science in Chemistry
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Jose Rene L. Micor
Co-adviser
Cleofas R. Cervancia
Committee Member
Banyuhay P. Serrano, Kristian Mark P. Caldo
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Abstract
The possible phenolic compounds present in Apis mellifera Linnaeus and Trigona biroi Friese propolis were identified using tandem liquid chromatography mass spectrometry and the total phenolic content of both propolis samples were determined using Folin-Ciocalteau assay. This study was able to identify some phenolic compounds present in A.mellifera and T.biroi propolis. There were 14 identified phenolic compounds present in A.mellifera propolis and 8 identified phenolic compounds present in T.biroi propolis. Flavonoids are the major group of phenolic compounds present in both samples. The results of this study strongly suggest the presence of luteolin-5-methyl-ether, pinobanksin-5, 7-dimethylether and Artepilin C in both A.mellifera and T.biroi propolis. The total phenolic content of methanolic and ethanolic extracts were determined. Results showed that the methanolic extracts of A.mellifera and T.biroi have only small difference in their total phenolic content. The T.biroi extract contained 2.44 ± 3.30x10-4 mg/g dry extract and A.mellifera propolis contained 2.49 ± 5.94 x10-3 mg/g dry extract. For the ethanolic extract, T.biroi propolis contained 5.38 ± 3.09x10-1 mg/g dry extract which is higher compared to A.mellifera propolis which contained 2.54 ± 3.17x10-4 mg/g dry extract. The total phenolic content of ethanolic extracts was found to be higher than the methanolic extracts for both A.mellifera and T. biroi.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2011 C4 L43
Recommended Citation
Lechuga, Jasmin A., "Preliminary study of phenolic compounds present in stingless bee (Trigona biroi Friese) and honey bee (Apis mellifera Linnaeus) propolis using tandem liquid chromatography/mass spectrometry" (2011). Undergraduate Theses. 1837.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1837
Document Type
Thesis