Preliminary study of phenolic compounds present in stingless bee (Trigona biroi Friese) and honey bee (Apis mellifera Linnaeus) propolis using tandem liquid chromatography/mass spectrometry

Date

4-2011

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Jose Rene L. Micor

Co-adviser

Cleofas R. Cervancia

Committee Member

Banyuhay P. Serrano, Kristian Mark P. Caldo

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Abstract

The possible phenolic compounds present in Apis mellifera Linnaeus and Trigona biroi Friese propolis were identified using tandem liquid chromatography mass spectrometry and the total phenolic content of both propolis samples were determined using Folin-Ciocalteau assay. This study was able to identify some phenolic compounds present in A.mellifera and T.biroi propolis. There were 14 identified phenolic compounds present in A.mellifera propolis and 8 identified phenolic compounds present in T.biroi propolis. Flavonoids are the major group of phenolic compounds present in both samples. The results of this study strongly suggest the presence of luteolin-5-methyl-ether, pinobanksin-5, 7-dimethylether and Artepilin C in both A.mellifera and T.biroi propolis. The total phenolic content of methanolic and ethanolic extracts were determined. Results showed that the methanolic extracts of A.mellifera and T.biroi have only small difference in their total phenolic content. The T.biroi extract contained 2.44 ± 3.30x10-4 mg/g dry extract and A.mellifera propolis contained 2.49 ± 5.94 x10-3 mg/g dry extract. For the ethanolic extract, T.biroi propolis contained 5.38 ± 3.09x10-1 mg/g dry extract which is higher compared to A.mellifera propolis which contained 2.54 ± 3.17x10-4 mg/g dry extract. The total phenolic content of ethanolic extracts was found to be higher than the methanolic extracts for both A.mellifera and T. biroi.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2011 C4 L43

Document Type

Thesis

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