Preliminary investigation on the applications of a carbon paste electrode modified with a protein mixture containing lectin

Date

4-2001

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Jose H. Santos

Abstract

ABSTRACT

ORBECIDO, AL MORETO B. College of Arts and Sciences, University of the Philippines Los Banos, April 2001. PRELIMINARY INVESTIGATION ON THE APPLICATIONS OF A CARBON PASTE ELECTRODE MODIFIED WITH A PROTEIN MIXTURE CONTAINING LECTIN.

Adviser: Dr. Jose H. Santos

A preliminary voltammetric study using a novel carbon paste electrode modified with an ammonium sulfate fraction of Artocarpus blancoi protein was conducted to determine the feasibility of its applications in chemical analysis. The study ventured on the analysis of heavy metals lead and mercury, and on the voltammetric discrimination among ABO blood groups. Cyclic and differential pulse-anodic stripping voltammetry were the electrochemical techniques used in the study. Optimization of parameters for the voltammetric applications were conducted and optimum response were observed with the following parameters: 0.1 M HCI supporting electrolyte, 25% (w/w) modifier composition, acidic to near neutral pH of application, accumulation time of 3 minutes and deposition time of 90 seconds. Analysis of heavy metal standard solutions showed that the electrode was capable of preconcentrating the analytes and effect current response during stripping. The electrode was found to have a sensitivity of 0.44 IAA/ppm and 0.11 μA/ppm for Pb(11) and Hg(II), respectively. Detection limb (LOD), based on a 3:1 signal-to-noise ratio, was 1.8 ppm for Pb(II) determinations while 3.3 ppm for lig(11). Results from the study on voltammetric discrimination among ABO blood groups were inconclusive and the presence of undefined variables in the experimental design used hindered the effective evaluation and interpretation of data gathered.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2001 C4 O73

Document Type

Thesis

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