Isolation and partial structure elucidation of a bioactive compound from the seeds of Swietenia macrophylla King

Date

6-1998

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evamarie P. Capareda

Abstract

TANGONAN, ANDREW ARGUELLES, University of the Philippines Los Banos, June 1998. Isolation and partial structure elucidation of a bioactive compound from the seeds of Swietenia macrophylla King.

Adviser: Evamarie P. Capareda, Ph.D.

Co-adviser: Evelyn B. Rodriguez, Ph.D

The chloroform extract of the seeds of Swietenia macrophylla King demonstrated toxicity against the brine shrimp (Artemia salina). A bioassay-guided fractionation using chromatographic techniques led to the isolation of a bioactive white powder, QZ3E. The LC50 of this isolate in the brine shrimp cytotoxicity assay was determined to be 67 ppm. Using the Plate Disc Method for anti-microbial assay, QZ3E was found to inhibit the growth of both Escherichia coli and Staphylococcus aureus. Analysis of spectral data (FTIR, UV, 13C- and 1H- NMR) obtained for compound QZ3E indicated the presence of a five membered a, ß unsaturated lactone in the structure.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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