A preliminary investigation of the use of diffraction- based sensing for the rapid detection of Papaya RingSpot Virus (PRSV) in Carica papaya Linn. Leaf samples.

Date

5-2011

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Milagros M. Peralta

Abstract

TONGCO, JOVALE VINCENT V, Institute of Chemistry, College of Arts and Sciences. University of the Philippines Los Banos, May 2011. A preliminary investigation of the use of diffraction — based sensing for the rapid detection of papaya ringspot virus (PRSV) in Carica papaya Linn. leaf samples.

Adviser: Dr. Milagros M. Peralta

Co-Adviser: Dr. Filomena C. Sta. Cruz

Advisory Committee Members: Dr. Marivic S. Lacsamana

Prof. Lou Serafin M. Lozada

Enzyme-linked immunosorbent assay (ELISA) and diffraction — based sensing (DBS) for detecting the presence papaya ringspot virus (PRSV) in papaya leaf samples were compared qualitatively. In both methods, the specificity of the antibody — antigen interaction between the PRSV capture antibody from AgdiaTM and papaya leaf extracts containing the virus was utilized for rapid detection of PRSV in infected plants.

Preliminary runs of leaf sap extracts from infected and healthy plants and control (PBS buffer only) confirmed the ability of DBS to detect the presence of PRSV by an increase in the intensity of the diffracted laser beam, thus increasing the potential difference of the photodiode reading. Infected samples yielded the highest photo-voltage difference at 0.9 rnV, healthy samples at 0.1 mV, and PBS buffer as negative control with 0.0 mV readings.

The optimization experiments were carried out using three configurations: antibody first (.AB - AG), antigen first (AG - AB), and sandwiched (AB — AG - AB) modes. Analyses of the results obtained using 20 plant samples showed that the two methods were comparable in their ability to detect the presence of the virus. However, DIIS had the added advantage of being a less expensive, more rapid, and simpler detection method compared to ELISA.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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