Isolation, partial characterization and identification of Rhizobacteria and efficacy of bacteria- produced auxin to enhance growth of corn (Zea mays)

Date

10-2013

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Jocelyn T. Zarate

Abstract

Efficacy of bacteria-produced auxin to enhance growth of corn was determined by isolating nitrogen-fixers on corn roots collected from four different sites and subjecting the isolates to IAA Assay through spectrophotometry. Top 5 auxin-producing microorganisms, referred to as isolate B4, B6, B9, B11 and C1, were identified as Klebsiellaspecies based on the 16s rRNA homology sequencing. The auxin activity of the isolates was tested through pot experiment which lasted for 1 week but results showed that even though the average plant height of the corn treated with isolates differ from the control, based on statistics the results were not significant so validation of the results was made through another pot experiment that lasted for 28 days. After 28 days, results showed that all the isolates have significant effects on the corn plants especially the mixed isolates in pellet form, which has the highest plant height value of 81.8 cm (25.7% increase) and longest root length value of 56.5 cm (53.5% increase). It can be concluded thatthe bacteria-produced auxin really has an effect on the plant height and root length of Zea mays and the isolates can be used as biofertilizersespecially the mixed isolates in pellet form.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 B4 /B38

Document Type

Thesis

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