Research internship on the comparative gizzard bacterial diversity and growth performance of broilers fed diets supplemented with antibiotic growth promoters and Bacillus subtilis /

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Amado A. Angeles

Abstract

The research internship was conducted to expose the intern in growing broilers and in profiling bacterial species by working on an experiment that determined the effect of diets with zinc bacitracin and chlortetracycline-based antibiotic growth promoters and Bacillus subtilis-based probiotics on the growth performance of broiler and bacterial diversity in the gizzard. Acquired techniques learned in the laboratory helped the intern attain the results of the study. Many DNA extraction protocols were undertaken and optimized to obtain better results. Quantity and quality check techniques were also employed. DNA amplification procedures were optimized to obtain the expected product size which was subjected to denaturing gradient gel electrophoresis (DGGE). Results showed that the bacterial diversity within and among the treatments qualitatively differed based on DGGE banding patterns. Statistical analysis also showed that there was no significant effect on the growth performance of broilers when zinc bacitracin and chlortetracycline-based antibiotic growth promoters and Bacillus subtilis-based probiotics were added on the diet. Through the internship, the intern learned and gained competence on molecular techniques as well as acquired associated work ethics needed for professional practice.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2015 A127 S89

Document Type

Thesis

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