Research internship on the comparative cecal bacterial diversity and growth performance of broilers fed diets supplemented with antibiotic growth promoters and probiotics

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Amado A. Angeles,

Abstract

The research internship aimed to familiarize the intern with different molecular biotechnology techniques such as DNA extraction, Polymerase Chain Reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) as well as knowing the production side of raising broilers. The study aimed to know the effects of varying diets: T1 (basal feed without AGP and probiotic), T2 (basal feed with AGP), T3 (basal feed with Bacillus subtilis) same as T4 and T6, T5 (basal feed with Enterococcus faecium) and T7 (basal feed with Enterococcus faecium, Birido bacterium and Lactobacillus sp. on production performance and bacterial diversity in the ceca of broilers. Two feeding trials were done in the study. For bacterial diversity analysis, three (3) replicates per treatment were used in DNA extraction and subjected to PCR to amplify the V3 to V5 of the 16S bacterial rDNA and to DGGE. Using ANOVA in CRD (P>0.5), AGP and probiotics showed insignificant results on the growth performance of broilers. PCR-DGGE results showed that there were 5 possible different species of bacteria present in the ceca of broilers. After the internship, the intern was able to learn various molecular biotechnology techniques for bacterial species diversity analysis and acquiring knowledge on raising broilers which can be applied by the intern in the animal industry.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2015 A127 /V37

Document Type

Thesis

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