DNA fingerprinting of resistant and susceptible abaca (Musa Textilis Nee) hybrids and parentals using simple sequence repeats (SSR), resistance gene analog (RGA) and AbTV and BBTV gene specific markers

Date

4-2012

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Lalusinn, Antonio C.

Abstract

Using 6 susceptible and 4 resistant parentals and hybrids of abaca, AbBTV and BBTV gene specific markers were used to verify the presence of AbBTV in these samples. S1 and S2 samples were positive for AbBTV, but all samples were negative for BBTV. In order to validate the presence of the AbBTV resistance gene in the samples, RGA primers were used. Abuab, BCF2-73 and BCF2-93 were positive for RGA1, while only BCF2-73 and BCF2-93 have the positive results for the RGA2. Other RGA primers are recommended for optimization. Simple sequence repeat (SSR) primers mMaCIR07, Mb1-12, mMaCIR22, mMaCIR39, mMaCIR150, and mMaCIR264 were screened to validate its association with resistance trait against AbBTV. Of the primers used, mMaCIR22 produced homozygous profile. The markers mMaCIR07, Mb1-12, mMaCIR39, and mMaCIR264 were observed to have a heterozygous profile with 2-band patterns for every primer. The mMaCIR150 marker was the most efficient to use because it produced a three-band patterns from the samples and having a PIC value of 0.708481. The data obtained were inputted to NTSYSsoftware for clustering under Jaccard’s similarity coefficient. Two clustering methods were used. The clustering method with the score of zero “0” produced resistant and susceptible groups.Thus, there is a possibility that some of the target sequence (regions) of the primers from banana do not exist in the abaca genome. Hence, no band will be produced for this absent region.

Language

English

Location

UPLB Main Library Special Collections Section

Call Number

Thesis

Document Type

Thesis

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