Method development and validation : determination of trace amount of lead in water using base-modified rice straw SPE column and anodic stripping voltammetry

Date

10-2013

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Mary Grace E. Guardian

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

A method for determining trace amount of lead in water employing base modified rice straw SPE columns and subsequent determination by ASV was developed and validated. Base-modified rice straw columns were prepared and its absorbing capability was compared to the commercially available Supelclean LC-Si Column. Using the optimized parameters for SPE elution and ASV measurements, the method was validated by assessing LOD, LOQ, accuracy, linearity, dynamic range, repeatability and intermediate precision. The calculated LOD and LOQ were 0.1700 ug L-1and 0.5000 ug L-1, respectively. Linearity of the method was established from the value of R2 and the plot of residuals. The linearity range of the method is 0.0 ? 50.0 𝜇g L-1 and a dynamic range of 0.50 ? 50.0 𝜇g L-1. The method was found to be accurate with percent recovery values of 82.65 % ± 7.85, 89.59 % ± 7.97 and 90.82 % ± 8.61 for a concentration of 5.0, 10.0 and 15.0 𝜇g L-1 respectively. Precision was expressed in terms of %RSD of the results. A % RSD of 6.67 % was obtained for its repeatability while 7.45 % was obtained for its intermediate precision.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 C42 /D44

Document Type

Thesis

This document is currently not available here.

Share

COinS