Method development and validation : determination of trace amount of lead in water using base-modified rice straw SPE column and anodic stripping voltammetry
Date
10-2013
Degree
Bachelor of Science in Chemistry
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Mary Grace E. Guardian
Restrictions
Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.
Abstract
A method for determining trace amount of lead in water employing base modified rice straw SPE columns and subsequent determination by ASV was developed and validated. Base-modified rice straw columns were prepared and its absorbing capability was compared to the commercially available Supelclean LC-Si Column. Using the optimized parameters for SPE elution and ASV measurements, the method was validated by assessing LOD, LOQ, accuracy, linearity, dynamic range, repeatability and intermediate precision. The calculated LOD and LOQ were 0.1700 ug L-1and 0.5000 ug L-1, respectively. Linearity of the method was established from the value of R2 and the plot of residuals. The linearity range of the method is 0.0 ? 50.0 𝜇g L-1 and a dynamic range of 0.50 ? 50.0 𝜇g L-1. The method was found to be accurate with percent recovery values of 82.65 % ± 7.85, 89.59 % ± 7.97 and 90.82 % ± 8.61 for a concentration of 5.0, 10.0 and 15.0 𝜇g L-1 respectively. Precision was expressed in terms of %RSD of the results. A % RSD of 6.67 % was obtained for its repeatability while 7.45 % was obtained for its intermediate precision.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2013 C42 /D44
Recommended Citation
De Guzman, Arvie Camille V., "Method development and validation : determination of trace amount of lead in water using base-modified rice straw SPE column and anodic stripping voltammetry" (2013). Undergraduate Theses. 2203.
https://www.ukdr.uplb.edu.ph/etd-undergrad/2203
Document Type
Thesis