Parametric study on chemical and enzymatic hydrolosis of alginate from Sargassum cristaefolium C.A. Agardh (Phaeophyta) for bioethanol production

Date

2011

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

Co-adviser

Irene G. Pajares, Milagrosa Goss

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Abstract

Parametric study for the chemical and enzymatic hydrolysis of alginate from seaweed, Sargassum cristaefolium was conducted to determine its potential for bioethanol production. The effect of time (1hr, 3hrs and 5hrs), temperature (60oC, 80oC, and 100 oC) and acid concentration (70%, 80%, and 90%) on the reducing sugar and uronic acid yield were determined for the chemical hydrolysis. It was found out that time has no significant effect on the reducing sugar yield but has significant effect on uronic acid yield. In terms of the effect of temperature, reducing sugar showed a decreasing trend with increasing temperature. For uronic acid, a peak value was observed at 80oC and further increase in temperature resulted in decreasing uronic acid yield. In terms of the effect of acid concentration, both reducing sugar and uronic acid exhibited a peak value at 80% acid concentration and further increase resulted in decreasing yields. Optimum chemical hydrolysis condition based on the highest amount of reducing sugar was found to be at 60oC, 80% acid concentration and 1hour. The effect of time (24hrs, 48hrs, and 72hrs) and temperature (37 oC, 40 oC and 45oC) were investigated during enzymatic hydrolysis. Results showed an increasing reducing sugar yield with increasing time whereas a decreasing reducing sugar yield was observed with increasing temperature. Optimum hydrolysis condition based on the highest reducing sugar was found to be at 37oC and 72hours.

The optimum conditions were evaluated on the extracted alginate. However, for enzymatic hydrolysis the condition applied was at 45oC and 72hours. Chemical hydrolysis yielded 0.0005 mg/ml reducing sugar while enzymatic hydrolysis yielded 0.9915 mg/ml reducing sugar. The highest amount was used to determine the bioethanol potential of the hydrolysates and was found to be too low to be considered for bioethanol production. Further studies for enzymatic hydrolysis were recommended as this gave quite meaningful results in the experiment.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Document Type

Thesis

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