Parametric study on hydrolysis of Laminarin extracted from Sargassum cristaefolium for hydrolysate preparation in bioethanol production

Date

2011

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

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Abstract

Laminarin was extracted from Sargassum cristaefolium using the modified method of Black in the extraction of laminarin and found to be 2.5438% (w/w) of the macroalgae. Crude laminarin underwent dilute acid hydrolysis for hydrolysate preparation in bioethanol production using hydrochloric and sulfuric acids, respectively. The highest sugar yield of 84.5438% was obtained with conditions of 0.1 M, 1000C, and 5 hr. for hydrochloric acid with reducing sugar concentration of 3.2611 mg/ml and 85.5175% with conditions of 0.1 M, 1000C, and 5 hr. for sulfuric acid with reducing sugar concentration of 3.2994 mg/ml, respectively. Effects of the parameters for hydrolysis (temperature, acid concentration, and reaction time) were determined using Statistical Analysis and Response Surface Methodology. An increase in the temperature during hydrolysis increases the reducing sugar concentration. Acid concentration had an inverse effect on the reducing sugar concentration while the reaction time does not significantly affect the hydrolysis process. Sulfuric acid appeared to be a more suitable acid in the hydrolysis as manifested by the results of the experiment.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Document Type

Thesis

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